Panel Series
Background: An anti-inflammatory effect of light obtained from light-emitting diodes (LEDs) has been discovered, however, limited ranges of wavelengths have been used and the action mechanism has been rarely demonstrated.
Objective: We sought to analyze the immunomodulatory effect of LED on Jurkat T cells and human T cells.
Methods: Jurkat T cells with/without stimulation were irradiated once or five times using seven ranges of LED wavelengths, from 415nm to 940nm. Cytotoxic effects were examined by an MTT assay. Changes in T cell-induced cytokines, including IL-2, IL-4, IL-10, IL-12 and IFN-gamma, and their upstream signaling molecules, ZAP-70 and PKCθ, were examined by real-time PCR, ELISA, and Western blot analysis.
