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The effect of low level laser irradiation (LLLI) was studied on cultured clonal bone cells, the RJC line. The bone cells were exposed to laser beams of various wavelengths (632.8, 635, 650, 670, 780, and 830 nm) using several energy densities. DNA synthesis in the treated cultures was evaluated by measuring the tritium-labelled thymidine uptake. A significant increase in DNA synthesis was observed at the wavelengths of 632.8, 635 and 830 nm, depending on the energy density level. A decrease was found at 780 nm. These findings suggest a possible therapeutic use of LLLI in the process of bone repair.
