Photoactivation of Cytochrome C Oxidase Activity in Japanese Quail Liver Mitochondria by Therapeutic Doses of Blue and Red LED Irradiation

The aim of the study was to determine the effect of blue (450 nm) and red (630 nm) LED irradiation with different energy exposure on the activity of mitochondrial cytochrome c oxidase. Methods. The object of the study was mitochondria isolated from the liver of the Japanese quail Coturnix japonica. Cytochrome c oxidase activity was measured by the oxidation rate of reduced tetramethyl-p-phenylenediamine in the presence of rotenone. Results. It was shown that irradiation of mitochondria with blue light with energy exposures not exceeding 4 J/cm² causes approximately 5-15% stimulation of cytochrome c oxidase activity, and doses greater than 5 J/cm² lead to inhibition of the enzyme activity. Irradiation of mitochondria with red light also has a stimulating effect (10-20%) on cytochrome c oxidase activity compared to non-irradiated samples. Conclusion. It is suggested that low-dose blue light irradiation may have similar therapeutic effects to red light in photobiomodulation.